Scaffold Design and Fabrication Techniques: A Comprehensive Guide for Biotechnology Students

Question 1

Which of the following is **NOT** a key consideration in designing a scaffold for tissue engineering applications?

Accepted Answer

Cost-effectiveness

Answer

Biocompatibility

Answer

Biodegradability

Answer

Mechanical strength

Question 2

Which fabrication technique is widely used to create scaffolds with precisely controlled microstructures and complex geometries?

Accepted Answer

3D printing

Answer

Electrospinning

Answer

Salt leaching

Answer

Solvent casting

Question 3

What is a key advantage of utilizing biodegradable polymers as scaffold materials?

Accepted Answer

They allow for gradual scaffold degradation as new tissue forms, matching the pace of tissue regeneration

Answer

They minimize the risk of adverse immune reactions

Answer

They enhance cellular adhesion and promote cell growth

Answer

They are inherently antimicrobial and prevent infections

Question 4

Which regulatory agency is responsible for approving tissue engineering products in the United States?

Accepted Answer

Food and Drug Administration (FDA)

Answer

Environmental Protection Agency (EPA)

Answer

National Institutes of Health (NIH)

Answer

Centers for Disease Control and Prevention (CDC)

Question 5

Which of the following is NOT a primary consideration in scaffold design for tissue engineering applications?

Accepted Answer

Aesthetics

Answer

Biocompatibility

Answer

Mechanical properties

Answer

Cost-effectiveness

Question 6

Which scaffold fabrication technique involves using a sacrificial template to create a porous structure?

Accepted Answer

Particulate leaching

Answer

Electrospinning

Answer

3D printing

Answer

Solvent casting

Question 7

What is a key advantage of using natural polymers for scaffold fabrication in tissue engineering?

Accepted Answer

Biodegradability

Answer

Low cost

Answer

High mechanical strength

Answer

Good cell adhesion

Question 8

What is the primary purpose of incorporating growth factors into scaffold design for tissue engineering?

Accepted Answer

Promote cell proliferation and differentiation

Answer

Enhance scaffold vascularization

Answer

Improve mechanical properties

Answer

Reduce scaffold degradation rate

Question 9

Which mechanical property is essential for scaffolds to resist external forces, maintain their structure, and provide a stable environment for tissue regeneration?

Accepted Answer

Stiffness

Answer

Flexibility

Answer

Porosity

Answer

Biodegradability

Question 10

How does porosity enhance the effectiveness of scaffolds in tissue regeneration?

Accepted Answer

Porosity allows nutrients and oxygen to reach cells, supporting their growth and tissue formation.

Answer

High porosity weakens the mechanical strength of scaffolds.

Answer

Porosity does not affect the compatibility of scaffolds with biological systems.

Answer

Low porosity is preferred for scaffolds used in weight-bearing applications.

Question 11

When choosing a scaffold material for tissue engineering, which factor is of utmost importance?

Accepted Answer

Biocompatibility

Answer

Mechanical strength

Answer

Cost-effectiveness

Question 12

Which of the following is NOT a desirable property of a scaffold for tissue engineering?

Accepted Answer

Corrosion resistance

Answer

Biocompatibility

Answer

Biodegradability

Answer

High mechanical strength

Question 13

What is the primary function of a sacrificial template in scaffold fabrication?

Accepted Answer

To remove unwanted material from the scaffold

Answer

To create a mold for casting the scaffold

Answer

To provide support during cell growth

Answer

To control the release of bioactive factors

Question 14

Which of the following techniques is most commonly used to create porous scaffolds with interconnected pores?

Accepted Answer

Gas foaming

Answer

Electrospinning

Answer

3D printing

Answer

Molding

Question 15

What is a key advantage of using natural polymers for scaffold fabrication?

Accepted Answer

They are typically biodegradable and biocompatible

Answer

They can be easily tailored to control their properties

Answer

They are inexpensive and readily available

Answer

They are suitable for all tissue engineering applications

Question 16

What is the main purpose of surface modification in scaffold fabrication?

Accepted Answer

To improve cell adhesion and proliferation

Answer

To increase scaffold degradation

Answer

To inhibit drug release

Answer

To hinder scaffold integration with host tissue

Question 17

Which scaffold fabrication technique involves depositing layers of biomaterial and cells to create a 3D structure?

Accepted Answer

3D printing

Answer

Electrospinning

Answer

Phase separation

Answer

Gas foaming

Question 18

What is a crucial property of an effective scaffold for tissue engineering?

Accepted Answer

Biocompatibility

Answer

Low production cost

Answer

Aesthetic appeal

Answer

High compressive strength

Question 19

Which of the following materials is not suitable for scaffold fabrication in tissue engineering?

Accepted Answer

Polystyrene

Answer

Chitosan

Answer

Polycaprolactone

Answer

Hydroxyapatite

Question 20

What is the primary purpose of incorporating a porogen into scaffold design?

Accepted Answer

To create interconnected pores for cell migration and nutrient transport

Answer

To facilitate scaffold sterilization

Answer

To enhance scaffold mechanical properties

Answer

To reduce scaffold degradation rate

Question 21

When selecting a scaffold fabrication method, which factor is essential to consider?

Accepted Answer

Desired scaffold architecture

Answer

Personal preferences of researchers

Answer

Availability of advanced equipment

Answer

Cost of the materials

Question 22

What is the primary objective of scaffold characterization?

Accepted Answer

To analyze scaffold properties and performance

Answer

To determine the optimal scaffold design parameters

Answer

To predict the in vivo behavior of the scaffold

Question 23

Which technique provides accurate measurements of scaffold porosity?

Accepted Answer

Mercury intrusion porosimetry

Answer

Atomic force microscopy

Answer

Scanning electron microscopy

Answer

Light microscopy

Question 24

How does scaffold stiffness affect tissue engineering outcomes?

Accepted Answer

It can influence cell differentiation, proliferation, and extracellular matrix production

Answer

It affects the rate of scaffold degradation

Answer

It determines the scaffold's ability to withstand mechanical forces

Answer

It has no impact on tissue engineering outcomes